Pre-school education, as a key stage on the path of children’s growth, plays a vital role in their overall development. Based on the independent sample t-test method, this paper explores the gender differences in preschool education. It also takes digital media education methods as an example, and utilizes Pearson correlation coefficient, linear regression model, and systematic clustering algorithm comprehensively to quantitatively assess the impact of education methods. The results of the study showed that there were extremely significant differences (P<0.01) in the five dimensions of language ability, creativity, social interaction ability, critical thinking ability, and independent learning ability between male and female toddlers, indicating that there are significant gender differences in preschool education effectiveness. The correlation coefficients between the frequency and duration of use of digital media education methods and language skills, creativity, social interaction skills, critical thinking skills, and independent learning skills ranged from 0.47 to 0.75, with significant positive correlations, and were associated with higher scores on each of the competencies as well as higher levels of satisfaction. This paper reveals in depth the gender differences in preschool education and the important role of digital media in preschool education, which is of great value for the optimization of teaching methods in preschool education.