The development of artificial intelligence has brought new development opportunities for modern enterprises, but employees present a certain degree of resistance to the introduction of AI technology. The author tries to dissipate employees’ resistance and improve their acceptance of AI through organizational training. After researching organizational training and employees’ perceived awareness of AI, organizational training and employees’ acceptance of AI are taken as antecedent and consequent factors to construct a structural equation research model of the two. The research hypotheses are proposed based on the theoretical study of the two. Regression analysis of the effect of organizational training on employees’ AI acceptance is conducted through structural equations. The regression results show that training investment, employee motivation and knowledge training in organizational training all have a significant positive effect on both employees’ AI perceived ease of use and AI perceived usefulness. Employee AI perceived ease of use and AI perceived usefulness have a positive effect on employee behavioral intention to use AI for knowledge creation and automation. Employees’ behavioral intention to use AI for knowledge creation will have a positive effect on AI for knowledge creation, and behavioral intention to use AI for automation will have a positive effect on AI for automation.