In order to improve the automation and intelligence level of underground fluid sampling, this paper proposes a kind of underground fluid automatic sampling device, and carries out the structural design of the sampling device, the control system design and the field experiment test. According to the action process and movement characteristics of the underground fluid stratified sampling device, the control system needs to use multiple electromagnetic control valves to control the switching of the oil circuit of different actuators respectively. In order to improve the control state and response speed of the stratified sampling device system, a fuzzy identification algorithm is chosen to identify the control model, and the MIMO robust generalized predictive controller is used as the robust adaptive controller of the system to realize the low-flow and low-disturbance acquisition of underground fluids at the same monitoring point and at different depths. In the field sampling, the average values of DO at sampling depths of 1m, 2m, 3m, 4m, and 5m for manual sampling, vertical sampling, and fuzzy adaptive device sampling under the 1-2 sampling plumb line were 7.98mg/L, 7.86mg/L, 8.25mg/L, 7.83mg/L, and 7.77mg/L, respectively. The deviation of dissolved oxygen content at the same sampling point in the three ways is small and the trend of change is consistent at different depths. It shows that the fuzzy adaptive stratified sampling device system designed in this paper can be applied to the sampling of subsurface fluids with dissolved oxygen as the detection target.