In higher education, physical education courses and ideological and political education are often carried out independently, lacking effective integration and interaction. This paper explores the informationization reform of physical education courses in universities and evaluates its impact on online ideological and political education. First, interdisciplinary integration and informationization methods are used to optimize the physical education course design and integrate ideological and political education content. Then, an online learning platform is constructed to break the limitations of time and space, encourage students to deeply understand the ideological and political education content in physical education teaching, and provide real-time feedback and personalized learning support. Multimedia technology is also adopted to enhance students’ understanding and internalization of sportsmanship and ideological and political education concepts. Students’ learning behavior and ideological and political education absorption in physical education courses are individually evaluated through data analysis. Finally, the profound impact is evaluated through experiments. The results indicate that the informationization reform of physical education courses in universities significantly improves students’ participation and knowledge mastery and effectively promotes the improvement of students’ ideological and political literacy, with the average score increasing by about 14%. These results provide strong empirical support for future teaching design and also provide valuable experience for further exploring the deep integration of physical education courses and online ideological and political education.