In a given graph , a set of vertices with an assignment of colors is a defining set of the vertex coloring of , if there exists a unique extension of the colors of to a -coloring of the vertices of . A defining set with minimum cardinality is called a smallest defining set (of vertex coloring) and its cardinality, the defining number, is denoted by . We study the defining number of regular graphs. Let be the smallest defining number of all -regular -chromatic graphs with vertices, and . Mahmoodian and Mendelsohn (1999) determined the value of for all , except for the case of . They showed that , for . They raised the following question: Is it true that for every , there exists such that for all , we have ?
Here we determine the value of for each in some congruence classes of . We show that the answer for the question above, in general, is negative. Also, for and the value of is determined except for one single case, and it is shown that .