In a given graph , a set of vertices with an assignment of colors is a defining set of the vertex coloring of , if there exists a unique extension of the colors of to a -coloring of the vertices of . A defining set with minimum cardinality is called a smallest defining set (of vertex coloring) and its cardinality, the defining number, is denoted by . Let be the smallest defining number of all -regular -chromatic graphs with vertices. Mahmoodian proved that, for a given and for all , if then . In this paper we show that for a given and for all and , .