For a finite group \(G\) and subsets \(T_1, T_2\) of \(G\), the Bi-Cayley digraph \(D = (V(D), E(D)) = D(G, T_1, T_2)\) of \(G\) with respect to \(T_1\) and \(T_2\) is defined as the bipartite digraph with vertex set \(V(D) = G \times \{0, 1\}\), and for \(g_1, g_2 \in G\), \(((g_1, 0), (g_2, 1)) \in E(D)\) if and only if \(g_2 = t_1 g_1\) for some \(t_1 \in T_1\), and \(((g_1, 1), (g_2, 0)) \in E(D)\) if and only if \(g_1 = t_2 g_2\) for some \(t_2 \in T_2\). If \(|T_1| = |T_2| = k\), then \(D\) is \(k\)-regular. In this paper, the spectra of Bi-Circulant digraphs are determined. In addition, some asymptotic enumeration theorems for the number of directed spanning trees in Bi-Circulant digraphs are presented.
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