Let \(A\) be the \((0,1)\)-adjacency matrix of a simple graph \(G\), and \(D\) be the diagonal matrix \(diag(d_1, d_2, \ldots, d_n)\), where \(d_i\) is the degree of the vertex \(v_i\). The matrix \(Q(G) = D + A\) is called the signless Laplacian of \(G\). In this paper, we characterize the extremal graph for which the least signless Laplacian eigenvalue attains its minimum among all non-bipartite unicyclic graphs with given order and diameter.