The Merrifield-Simmons index of a graph is defined as the number of subsets of the vertex set, in which any two vertices are non-adjacent, i.e., the number of independent vertex sets of . A tree is called an -leaf tree if it contains vertices with degree one. In this paper, we obtain the smallest Merrifield-Simmons index among all trees with vertices and exactly six leaves, and characterize the corresponding extremal graph.